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The ST3 jack is rather close to the USB port, and when QuDrive is in use there have been reports of noise being picked up by ST3 – effectively interference from the USB transfer activity to the ST3 analogue pickup.
If you have something plugged into the ST3 jack then this effectively moves the ST3 input point further from the USB port, and that can reduce/eliminate the interference – this shouldn’t be relevant in your case.
IF the ST3 source isn’t set to USB then this could explain the noise.
The Global USB source will also need to be QuDrive, not USB-B.
It’s also worth noting that there used to be a requirement for wav files to be 48KHz/24 bit – I’m pretty sure this has been relaxed in recent firmware versions – but check if you need/want a firmware update, and the precise format of the files as well.